A sandwich transfer enzyme immunoassay for salmon calcitonin: Determination of the bioavailability of intranasal salmon calcitonin in human
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چکیده
منابع مشابه
Vascular and Renal Actions of Salmon Calcitonin
When freshwater eels were given either 5 or 15 i.u. calcitonin kg" body weight, there was an increase in dorsal aortic blood pressure as well as a rise in urine volume, glomerular filtration rate and electrolyte excretion rate, 1 h after injection. None of these changes was shown by seawater-adapted eels. When freshwater-adapted eels received 15 i.u. calcitonin kg" body weight there were signif...
متن کاملSensitive time-resolved fluoroimmunoassay of salmon calcitonin.
A two-site assay was developed by use of the "dissociation and enhancement lanthanide fluoroimmunoassay" (DELFIA) technique for determination of salmon calcitonin (SCT) in serum after administration to osteoporotic patients. Polyclonal antibodies were produced in rabbits immunized with SCT coupled to ovalbumin. After affinity purification, the antibodies were used both as immobilized capture an...
متن کاملSalmon calcitonin: conformational changes and stabilizer effects
The therapeutic activity of peptides or protein drugs is highly dependent on their conformational structure. The protein structure is flexible and responds to external conditions, which may compromise the protein’s native conformation and influence its physical and chemical stability. The physical and chemical stability of peptides or protein drugs are important characteristics of biopharmaceut...
متن کاملIdentification of key components in the irreversibility of salmon calcitonin binding to calcitonin receptors.
This study investigates the poor reversibility of salmon calcitonin (sCT) binding to rat and human calcitonin receptors. Efficacy of CT and analogue peptides in (125)I-sCT binding competition and cAMP assays was compared with the dissociation kinetics of (125)I-labelled peptides. Assessment was performed on cells stably expressing either rat or human calcitonin receptors. Dissociation kinetics ...
متن کاملProlonged Calcitonin Receptor Signaling by Salmon, but Not Human Calcitonin, Reveals Ligand Bias
Salmon calcitonin (sCT) and human calcitonin (hCT) are pharmacologically distinct. However, the reason for the differences is unclear. Here we analyze the differences between sCT and hCT on the human calcitonin receptor (CT(a)R) with respect to activation of cAMP signaling, β-arrestin recruitment, ligand binding kinetics and internalization. The study was conducted using mammalian cell lines he...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 1997
ISSN: 0887-8013,1098-2825
DOI: 10.1002/(sici)1098-2825(1997)11:6<380::aid-jcla13>3.0.co;2-s